CHM363
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Syllabus

Professor: Terese M. Wignot

267 Stark Learning Center
(570) 408-4627
wignot@wilkes.edu

Syllabus

Course Information

Class Hour: Monday 2:00-4:50; Please note time may be required outside of the regularly scheduled class hours

Location: SLC 275

Text: "Experiments in Biochemistry;A Hands on Approach" by Shaawn O. Farrell and Lynn E. Taylor, 2nd edition, Thomson Brooks/Cole ISBN 0-495-01317-X. Available in the bookstore.

Major Objectives:Students in all chemistry courses are expected:
A1. To demonstrate proficiency in analysis, organization, interpretation, and presentation of chemical data.
A2. To express chemical concepts with quantitative relationships and to interpret the results obtained from the use of these quantitative relationships in terms of the chemical concepts conveyed in this format.
A3. To use written communication in a cogent and coherent form that demonstrates understanding of chemical concepts.
A4. To develop critical thinking and problem-solving skills in synthesizing information.
A5. To appreciate the relevance of chemistry to everyday life.
A6. To recognize that the various areas of chemistry are interrelated and require integration of basic chemical principles, including chemical formulae and nomenclature, chemical reactions and stoichiometry, chemical equilibria and acid-base theory, and molecular structure.

Course Objective: Students in this courses are expected:
B1. To demonstrate an understanding and use of basic biochemical techniques.
B2. To demonstrate an understanding of the proper technique and application of protein purification including but not limited too heat denaturation, salting out, and dialysis.
B3. To demonstrate an understanding of the proper technique and application of chromatographic techniques including but not limited to size exclusion and affinity chromatography.
B4. To demonstrate proficiency in gathering, analysing, organization, interpretation, and presentation of enzyme kinetic data through spectrophotometric methods including but not limited to total protein assay and enzyme activity assays.
B5. To demonstrate an understanding of the proper technique and application of electrophoretic techniques including but not limited to SDS-PAGE and IEF.
B6. To demonstrate an understanding of the proper technique and application of western blotting techniques.
B7. To keeping a proper scientific laboratory notebook and converting laboratory data into a formal report.

Chemistry 363 is an independent 1-credit course. Your grade will be based upon a notebook (40%), a formal report (30%), peer review comments (10%), quizzes (10%),and participation (10%). Quizzes will be given periodically. Everyone is expected to come to lab prepared. Notebooks and the formal report will follow the formats listed below and a more detailed description of the grading will also be outlined. Time permitting; a practical lab final or written lab final will be given. ALL SAFETY rules are to be followed.

Percentage	Grade
>90	4.0
85-89	3.5
80-84	3.0
75-79	2.5
70-74	2.0
65-69	1.5
60-64	1.0
<60	0

All point distributions and cut offs are subject to change at the discretion of the instructor.